TB-500 (Thymosin Beta-4) Dosage Guide: How Much Should You Take? (2026)

Key Takeaways

• Standard Dosage Range: TB-500 is typically administered in doses ranging from 2 mg to 5 mg per week, depending on the specific use case and patient needs.
• Administration Method: The most common route of administration is subcutaneous injection, though other forms are under investigation.
• Cycle Length: Typical cycles last from 4 to 6 weeks, with maintenance doses potentially continuing thereafter.
• Regulatory Status: TB-500 is not FDA-approved and is available for research purposes only.
• Medical Supervision: Always consult a healthcare provider before starting TB-500 to ensure safe and effective use.

How TB-500 (Thymosin Beta-4) Works in the Body

TB-500, a synthetic derivative of Thymosin Beta-4, is known for its potential in promoting tissue repair and regeneration. This peptide operates by modulating the β-actin/G-actin cycle, which is crucial for cell migration, angiogenesis, and actin polymerization. The regulation of these processes is essential for wound healing and cellular motility.

The pharmacokinetics of TB-500 reveal a relatively short half-life, necessitating frequent dosing to maintain therapeutic levels. Bioavailability varies depending on the administration route, with subcutaneous injections providing more direct access to systemic circulation compared to other forms.

"The peptide segment (17)LKKTETQ(23) is the active site within the protein thymosin β(4) responsible for actin binding, cell migration and wound healing" (PMID 23084823).

Clinical studies, such as those referenced in PMID 22962027, emphasize the peptide's potential but also highlight the need for further research to fully understand its effects and optimal dosing strategies.

Standard TB-500 (Thymosin Beta-4) Dosage Ranges (By Form)

Subcutaneous Injection

• Dosage Range: 2 mg to 5 mg per week
• Frequency: Typically administered 2-3 times per week
• Bioavailability: High, with direct absorption into the bloodstream
• Commonality: Most prevalent due to ease of administration and effective absorption

Intramuscular Injection

• Dosage Range: Similar to subcutaneous, though less commonly used
• Frequency: 2-3 times per week
• Bioavailability: Comparable to subcutaneous but may vary based on muscle mass and injection technique

Oral and Other Forms

• Research Stage: Currently under investigation with limited data on efficacy and bioavailability
• Bioavailability: Generally lower due to digestive breakdown

Subcutaneous injections are the most common form due to their straightforward administration and reliable absorption profile.

TB-500 (Thymosin Beta-4) Dosage by Use Case

Wound Healing

• Typical Dosage Range: 2-5 mg per week
• Administration Method: Subcutaneous injection is preferred
• Cycle Length: 4-6 weeks, with potential for maintenance dosing
• Clinical Reference: Studies suggest enhanced epithelialization and reduced inflammation (PMID 23084823)

Musculoskeletal Injuries

• Typical Dosage Range: 2-4 mg per week
• Administration Method: Subcutaneous injection
• Cycle Length: 4-8 weeks, depending on the severity of the injury
• Practical Guidance: Many clinics recommend starting at the lower end of the dosing range and adjusting based on response

Inflammation Reduction

• Typical Dosage Range: 2-3 mg per week
• Administration Method: Subcutaneous injection
• Cycle Length: 4-6 weeks
• Clinical Reference: Anecdotal reports and preclinical studies suggest anti-inflammatory benefits

How Long Should You Take TB-500 (Thymosin Beta-4)?

Typical treatment cycles for TB-500 last between 4 to 6 weeks. During week 1, patients may begin to notice subtle changes, with more pronounced effects often reported by weeks 2 to 4. By week 6, many patients experience the full therapeutic benefits, though individual responses can vary.

Tapering off is not typically required, but ongoing maintenance doses may be considered for chronic conditions. Discontinuation should be guided by clinical outcomes and professional advice.

Administration Protocol: How to Take TB-500 (Thymosin Beta-4)

Injection Technique

• Needle Gauge: 29-31 gauge is commonly used for subcutaneous injections
• Injection Sites: Rotate between the abdomen, thigh, and upper arm to reduce site irritation
• Rotation Schedule: Alternate sites with each injection to promote healing

Storage Requirements

• Temperature: Store vials in a refrigerator at 2-8°C (36-46°F)
• Light Sensitivity: Protect from light to maintain stability
• Reconstituted Stability: Use within 7 days when stored properly

Reconstitution Instructions

• Diluent: Use bacteriostatic water for injection
• Process: Gently swirl the vial to mix; avoid shaking to prevent denaturation

For a detailed reconstitution guide, refer to our reconstitution guide.

Stacking TB-500 (Thymosin Beta-4) for Better Results

TB-500 and BPC-157

• Combination: Used for enhanced tissue repair and anti-inflammatory effects
• Dosing Synergy: TB-500 at 2-5 mg per week, BPC-157 at 200-500 mcg per day
• Common Protocols: Often used in protocols targeting musculoskeletal injuries

TB-500 and IGF-1 LR3

• Combination: Aimed at promoting muscle growth and repair
• Dosing Synergy: TB-500 at 2-4 mg per week, IGF-1 LR3 at 20-40 mcg per day
• Clinical Protocols: Utilized in recovery and regeneration-focused protocols

For more information on these peptides, visit the BPC-157 and IGF-1 LR3 pages.

Factors That Affect Your TB-500 (Thymosin Beta-4) Dosage

Several factors can influence the appropriate dosage of TB-500, including:

• Body Weight: Heavier individuals may require higher doses to achieve therapeutic effects.
• Age: Older adults may have altered metabolism affecting peptide clearance.
• Treatment Goals: Specific objectives, such as wound healing or inflammation reduction, may dictate dosing.
• Kidney/Liver Function: Impaired function can affect peptide metabolism and clearance.
• Individual Response: Variability in response necessitates personalized dosing adjustments.

Healthcare providers tailor dosing based on these factors to optimize outcomes and minimize risks.

Common Dosing Mistakes to Avoid

1. Dosing Too High/Too Low: Follow recommended ranges to avoid adverse effects or suboptimal results.
2. Inconsistent Timing: Maintain regular dosing schedules to ensure stable peptide levels.
3. Wrong Injection Site for the Goal: Use appropriate sites to maximize efficacy and comfort.
4. Not Accounting for Regulatory Status: Remember TB-500 is for research use only; consult professionals.
5. Poor Storage/Handling: Proper storage is essential to maintain peptide integrity and effectiveness.

What the Evidence Does Not Show

While TB-500 shows promise in preclinical studies, significant gaps remain in the evidence:

• Long-Term Safety: Limited data on the long-term effects of TB-500 use.
• Human Trials: Most studies are preclinical or anecdotal; rigorous human trials are needed.
• Dose-Response Data: More research is needed to establish precise dose-response relationships.

These limitations underscore the importance of cautious and informed use under professional guidance.

FAQ — Your Top TB-500 (Thymosin Beta-4) Dosage Questions Answered

Best time of day to take it?

TB-500 can be administered at any time of day, but consistency is key. Choose a time that fits your routine.

What if you miss a dose?

If you miss a dose, take it as soon as you remember unless it's close to the next scheduled dose. Do not double up.

Can men and women use the same dose?

Yes, dosing is generally similar for men and women, but individual factors may necessitate adjustments.

Can you stack it with other peptides?

Yes, TB-500 is often stacked with peptides like BPC-157 for enhanced effects. Consult a provider for tailored protocols.

How long until you see results?

Initial effects may be noticed within 1-2 weeks, with full benefits typically observed by week 4-6.

Do you need to cycle off?

Cycling off is not typically required, but maintenance dosing may be adjusted based on clinical outcomes.

Is a loading dose needed?

A loading dose is not generally recommended; start with standard dosing and adjust as needed.

How to adjust dose if side effects occur?

If side effects occur, consult your provider. They may suggest dose adjustments or discontinuation.

Conclusion — Finding the Right Dosage for You

TB-500 (Thymosin Beta-4) offers potential benefits for tissue repair and regeneration. Determining the right dosage involves considering individual factors and therapeutic goals. Always engage with a healthcare provider to ensure safe and effective use of TB-500. For further information, explore our peptide therapy guides and use our clinic finder to locate a qualified provider near you.

Medical Disclaimer: This content is for informational purposes only and does not constitute medical advice. Dosage information is compiled from published research and clinical protocols. Consult a licensed healthcare provider before starting any peptide therapy. Use our clinic finder to locate a qualified provider near you.