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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

DSIP Dosing Protocol: 10 mg Vial — Extended Sleep Protocol Guide

DSIP 10 mg vial guide — 14-week nightly supply at 100 µg/night, ideal for chronic insomnia protocols.

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Quickstart highlights

Chronic sleep insufficiency is associated with elevated cortisol, impaired immune function, and accelerated cognitive aging.

  • Concentration: 2 mg/mL (10 mg vial + 5 mL bacteriostatic water, reconstitute in 2.5 mL portions).
  • At 2 mg/mL: 100 µg = 5 units per nightly injection.
  • At 100 µg/night and 28-day reconstitution window: 28 doses per reconstitution event.
  • Reconstitute 2.5 mL (5 mg) portions twice over 56 days to cover a 12-week protocol.
  • Refrigerate; use reconstituted solution within 28 days.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-2251.25Once nightly25 µg — 1.25 units; starting dose; 10 mg vial provides 400 doses at this level
3-141005Once nightly100 µg — 5 units; one 10 mg vial provides 100 nightly doses (14 weeks at once-nightly)

Reconstitution steps

  1. Draw 5 mL bacteriostatic water (two 2.5 mL draws); inject slowly down the vial wall.
  2. Swirl gently until dissolved; do not shake.
  3. Final concentration: 2 mg/mL. Label with date; refrigerate at 2–8 °C. Use within 28 days.
  4. At 100 µg/night, 28-day window = 28 doses per reconstitution (28 nights). Reconstitute 2.5 mL portions for efficient use within the window.

Supplies needed

12-week plan

  • 1 vial
  • 84 syringes
  • 5 mL bac water
  • 84 alcohol swabs
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Protocol overview & cycle notes

Provide an extended 12+ week nightly DSIP sleep protocol from a single 10 mg vial, covering both assessment and maintenance phases for chronic insomnia or poor sleep quality management.

Cycle length: 12 weeks on.

Off-cycle: 4 weeks off; log PSQI sleep quality before resuming.

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days. Reconstitute 2.5 mL (5 mg) portions to stay within the window — the 10 mg vial lyophilized portion allows partial reconstitution.

Injection & tracking tips

  • Reconstitute 2.5 mL per cycle (5 mg = 50 nightly 100 µg doses) — use within 28 days, then reconstitute the remaining 2.5 mL.
  • The 10 mg vial reduces the number of new vials needed over a 12-week protocol from 2 to 1.
  • Stack with Selank (subcutaneous, afternoon) for anxiety + sleep quality synergy without combining in the same syringe.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • PSQI sleep quality questionnaire weekly throughout the 12-week protocol.
  • Track wearable sleep data (REM and deep sleep percentages) nightly.
  • Log morning cortisol symptoms weekly (anxiety on waking, fatigue, mood).
Log your cycle in the calculator →

How this works & references

Chronic sleep insufficiency is associated with elevated cortisol, impaired immune function, and accelerated cognitive aging. DSIP's dual mechanism — direct delta-wave sleep promotion and HPA axis dampening — addresses both structural sleep architecture and stress-mediated nighttime cortisol elevation. The 10 mg vial, reconstituted in 2.5 mL portions, provides two 28-night reconstitution cycles of 100 µg nightly dosing from a single lyophilized vial.

Sources

  • Source: Schoenenberger GA, Monnier M — DSIP characterization. Proc Natl Acad Sci. 1977;74(3):1282-6
  • Source: Schneider-Helmert D et al. — DSIP in chronic insomnia. Eur Neurol. 1985

Frequently asked questions

Is the 10 mg vial significantly better than two 5 mg vials for this protocol?
The 10 mg lyophilized vial allows partial reconstitution: reconstitute 2.5 mL (5 mg), use for 28 nights, then reconstitute the remaining 2.5 mL. This maintains potency of the unused dry peptide better than storing a partially used 5 mg vial in solution. Total convenience is comparable; the 10 mg vial may offer better potency preservation.
Can DSIP be combined with melatonin?
Yes — melatonin (0.5–3 mg) addresses circadian timing; DSIP addresses sleep architecture quality. Combining both targets different aspects of sleep: circadian onset (melatonin) and delta-wave depth (DSIP). No adverse interactions are known. Many sleep optimization protocols use both.
Does DSIP work for jet lag?
DSIP may reduce jet lag by normalizing the cortisol rhythm disrupted by rapid time-zone shifts. Unlike melatonin (which primarily advances sleep timing), DSIP promotes sleep quality regardless of circadian timing, potentially helpful during the adaptation period of 3–5 days. No published jet lag-specific studies exist.
How long does the sleep-improving effect persist after stopping DSIP?
Anecdotal reports suggest sleep quality improvements persist for 2–4 weeks after stopping a 12-week DSIP course — potentially due to residual HPA axis normalization and sleep habit consolidation. Return to baseline sleep quality after 4+ weeks suggests need for another cycle.
Is there a maximum nightly dose of DSIP?
The human trial by Schneider-Helmert used 30 nmol/kg IV (approximately 250 µg for a 70 kg person). Subcutaneous compounding protocols use 25–100 µg nightly. Doses above 200 µg have not been formally studied in human insomnia protocols; higher doses may cause transient hypotension in sensitive individuals.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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