AICAR Dosage Guide: How Much Should You Take? (2026)

Key Takeaways

• Standard Dosage Range: AICAR is typically dosed between 10-50 mg/kg body weight, depending on the administration method and therapeutic goals.
• Administration Method: Commonly administered subcutaneously or intramuscularly due to bioavailability; oral administration is less effective.
• Typical Cycle Length: Often used in cycles of 4-6 weeks, with adjustments based on clinical response and goals.
• Regulatory Status: AICAR is not FDA-approved and is available for research purposes only.
• Medical Supervision: Professional guidance is crucial to tailor dosing and monitor for side effects.

How AICAR Works in the Body

AICAR (5-Aminoimidazole-4-carboxamide ribonucleotide) is a research compound known for its role in activating AMP-activated protein kinase (AMPK). This enzyme plays a critical role in cellular energy homeostasis, influencing processes such as mitochondrial biogenesis, fatty acid oxidation, and glucose metabolism. Understanding these mechanisms is essential for determining appropriate dosing.

AICAR's mechanism of action involves mimicking adenosine monophosphate (AMP), thus activating AMPK. This activation leads to increased fatty acid oxidation and improved glucose uptake in skeletal muscle, which can be beneficial in metabolic regulation. The compound's half-life and bioavailability are influenced by its administration route, impacting how often and in what doses it should be given.

Several studies have explored AICAR's effects:

"MOTS-c, through the Folate-AICAR-AMPK pathway, influences energy metabolism and insulin resistance" (PMID 36670507).

"AICAR inhibits inflammation and promotes mitochondrial function, impacting oxidative stress" (PMID 36048820).

These findings highlight the importance of precise dosing to maximize therapeutic benefits while minimizing potential risks.

Standard AICAR Dosage Ranges (By Form)

Subcutaneous Administration

• Dosage Range: 10-20 mg/kg body weight per day
• Frequency: Once daily
• Bioavailability: High, due to direct absorption into the bloodstream
• Commonality: Preferred for consistent plasma levels and ease of administration

Intramuscular Administration

• Dosage Range: 15-25 mg/kg body weight per day
• Frequency: Once daily
• Bioavailability: Comparable to subcutaneous, with slightly slower absorption
• Commonality: Used when subcutaneous sites are not viable

Oral Administration

• Dosage Range: 50 mg/kg body weight per day
• Frequency: Once or twice daily
• Bioavailability: Low, due to first-pass metabolism
• Commonality: Less common due to reduced efficacy

Comparison

Subcutaneous and intramuscular routes are preferred over oral administration due to higher bioavailability and more reliable pharmacokinetics. The choice between subcutaneous and intramuscular may depend on patient preference and specific clinical scenarios.

AICAR Dosage by Use Case

Metabolic Regulation

• Typical Dosage Range: 10-20 mg/kg body weight per day
• Administration Method: Subcutaneous
• Cycle Length: 4-6 weeks
• Clinical Reference: Studies indicate improved glucose metabolism and fatty acid oxidation (PMID 36670507).
• Practical Guidance: Many clinics recommend starting at the lower end of the range, adjusting based on response.

Energy Regulation

• Typical Dosage Range: 15-25 mg/kg body weight per day
• Administration Method: Intramuscular
• Cycle Length: 6 weeks
• Clinical Reference: Evidence supports enhanced mitochondrial function and energy homeostasis (PMID 36048820).
• Practical Guidance: Clinical protocols typically involve monitoring energy levels and adjusting doses accordingly.

Neurological Conditions

• Typical Dosage Range: 20-30 mg/kg body weight per day
• Administration Method: Subcutaneous or intramuscular
• Cycle Length: 4-8 weeks
• Clinical Reference: AICAR's role in neuronal development and differentiation is under investigation (PMID 29634397).
• Practical Guidance: Dosages may vary significantly based on individual neurological needs and clinical trials.

How Long Should You Take AICAR?

Typical AICAR cycles range from 4 to 6 weeks, with some protocols extending to 8 weeks based on therapeutic goals and patient response. During the initial week, patients may experience increased energy levels and improved metabolic markers. By weeks 2-4, many report enhanced endurance and metabolic efficiency. After 6 weeks, the benefits plateau, and tapering may be necessary to prevent potential side effects.

Clinical trials suggest that continuous use beyond recommended cycles does not significantly enhance benefits and may increase the risk of adverse effects. Therefore, cycling off AICAR is advised, with a rest period equal to the cycle length before resuming treatment.

Administration Protocol: How to Take AICAR

Injection Technique

• Needle Gauge: 25-30 gauge for subcutaneous; 22-25 gauge for intramuscular
• Injection Sites: Rotate between abdomen, thighs, and upper arms
• Rotation Schedule: Change sites with each injection to minimize tissue irritation

Oral Administration Timing

• Timing: Best taken on an empty stomach to enhance absorption

Storage Requirements

• Temperature: Store at room temperature, away from direct sunlight
• Light Sensitivity: Protect from light to maintain potency
• Reconstituted Stability: Use within 24 hours if reconstituted

Reconstitution Instructions

Refer to our reconstitution guide for detailed instructions on preparing AICAR for injection.

Step-by-Step Administration Process

1. Wash hands thoroughly and gather supplies.
2. Prepare the injection site with an alcohol swab.
3. Draw the prescribed dose into the syringe.
4. Administer the injection at a 45-degree angle for subcutaneous or 90-degree for intramuscular.
5. Dispose of the needle safely and record the injection site and time.

Stacking AICAR for Better Results

AICAR and BPC-157

• Combination: Used for enhanced tissue repair and metabolic function
• Dosing Synergy: AICAR 10-20 mg/kg with BPC-157 200-500 mcg daily
• Common Protocols: Often used in injury recovery protocols

AICAR and TB-500

• Combination: Promotes muscle recovery and reduces inflammation
• Dosing Synergy: AICAR 15-25 mg/kg with TB-500 2-5 mg weekly
• Common Protocols: Employed in athletic performance enhancement

AICAR in the "Wolverine Protocol"

• Combination: AICAR with IGF-1 LR3 for muscle growth
• Dosing Synergy: AICAR 20 mg/kg with IGF-1 LR3 50-100 mcg daily

Factors That Affect Your AICAR Dosage

Several factors influence AICAR dosing, including body weight, age, treatment goals, kidney/liver function, and other medications. Healthcare providers tailor dosages to individual needs, ensuring optimal efficacy and safety. For instance, younger patients or those with higher metabolic rates may require adjusted dosages to achieve desired outcomes.

Common Dosing Mistakes to Avoid

1. Dosing Too High/Too Low: Always follow prescribed dosages to avoid adverse effects or diminished efficacy.
2. Inconsistent Timing: Maintain a regular dosing schedule to ensure stable plasma levels.
3. Wrong Injection Site for the Goal: Choose appropriate sites to maximize absorption and minimize discomfort.
4. Not Accounting for Regulatory Status: Remember that AICAR is research-only and not FDA-approved.
5. Poor Storage/Handling: Improper storage can degrade the compound, reducing effectiveness.

What the Evidence Does Not Show

While AICAR has shown promise in preclinical studies, there is limited long-term safety data in humans. Most evidence comes from animal studies or early-phase trials, highlighting the need for further research. Additionally, dose-response data is not fully established, necessitating cautious dosing under professional supervision.

FAQ — Your Top AICAR Dosage Questions Answered

Best time of day to take it
AICAR is typically administered in the morning to align with natural metabolic rhythms.

What if you miss a dose
If you miss a dose, take it as soon as you remember unless it's close to your next scheduled dose. Do not double up.

Can men and women use the same dose
Dosing is generally based on body weight, not gender, though individual responses may vary.

Can you stack it with other peptides
Yes, AICAR is often stacked with peptides like BPC-157 and TB-500 for synergistic effects.

How long until you see results
Many patients report noticeable effects within 1-2 weeks, though full benefits may take longer.

Do you need to cycle off
Yes, cycling off after 4-6 weeks is recommended to prevent tolerance and side effects.

Is a loading dose needed
Loading doses are generally not required; standard dosing protocols are sufficient.

How to adjust dose if side effects occur
If side effects occur, consult your provider to adjust the dosage safely.

Conclusion — Finding the Right Dosage for You

Determining the right AICAR dosage involves considering individual factors and therapeutic goals. Professional supervision ensures safe and effective use, tailored to your needs. Explore our peptide encyclopedia for more information on AICAR and related compounds, and use our clinic finder to locate a qualified provider near you.

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Medical Disclaimer: This content is for informational purposes only and does not constitute medical advice. Dosage information is compiled from published research and clinical protocols. Consult a licensed healthcare provider before starting any peptide therapy. Use our clinic finder to locate a qualified provider near you.