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MyPeptideMatch
Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

AOD-9604 Dosing Protocol: 10 mg Vial — Extended Fat Loss Protocol Guide

AOD-9604 10 mg vial guide — 20-day supply at 500 µg/day, ideal for extended visceral fat reduction cycles with reduced reconstitution frequency.

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Quickstart highlights

The 10 mg AOD-9604 vial provides approximately 20 daily doses at 500 µg — the Phase 2 human dose that demonstrated significant visceral fat reduction.

  • Concentration: 2 mg/mL (10 mg vial + 5 mL bacteriostatic water, reconstitute 2.5 mL at a time).
  • At 2 mg/mL: 500 µg = 25 units per morning fasted injection.
  • One 10 mg vial provides ~20 doses at 500 µg — approximately 3 weeks at once-daily dosing.
  • Reconstitute in 2.5 mL portions (5 mg each) for two 14-day reconstitution cycles.
  • No IGF-1 effect — suitable for patients with IGF-1-sensitive conditions.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-430015Once daily (morning, fasted)300 µg — 15 units; starting dose; 10 mg vial provides ~33 doses at this level
5-1250025Once daily (morning, fasted)500 µg — 25 units; maintenance dose; ~20 doses per vial

AOD-9604 Dosage Calculator

This free peptide calculator shows the exact insulin-syringe units to draw for AOD-9604. Enter your vial size, bacteriostatic water and target dose — it’s pre-loaded with common AOD-9604 reconstitution defaults, so you can adjust any field to match your own vial.

Your free AOD-9604 tracker

  • Dose reminders
  • Injection logging
  • Cycle progress
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Reconstitution steps

  1. Draw 5 mL bacteriostatic water (two 2.5 mL draws); inject slowly down the vial wall.
  2. Swirl gently until dissolved; do not shake.
  3. Final concentration: 2 mg/mL. At 2 mg/mL: 300 µg = 15 units, 500 µg = 25 units.
  4. Label with date; refrigerate at 2–8 °C. Use within 28 days.

Supplies needed

12-week plan

  • 2 vials
  • 84 syringes
  • 10 mL bac water
  • 84 alcohol swabs
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Protocol overview & cycle notes

Achieve extended visceral fat reduction protocol from the 10 mg AOD-9604 vial using partial reconstitution, targeting abdominal adiposity without IGF-1 elevation over a 12-week daily injection cycle.

Cycle length: 12 weeks on.

Off-cycle: 8 weeks off; assess body composition (DEXA, waist circumference) before resuming.

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days.

Injection & tracking tips

  • Morning fasted injection (30–60 minutes before eating or coffee) maximizes lipolytic effect.
  • Reconstitute 2.5 mL at a time (5 mg = 10 doses at 500 µg) to stay within the 28-day window.
  • Combine with aerobic exercise 1–2 hours after injection for enhanced fatty acid oxidation.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • DEXA body composition scan at baseline and 8 weeks.
  • Track waist and hip circumference weekly.
  • Log fasting glucose weekly — AOD-9604 is safe for diabetic patients.
Log your cycle in the calculator →

How this works & references

The 10 mg AOD-9604 vial provides approximately 20 daily doses at 500 µg — the Phase 2 human dose that demonstrated significant visceral fat reduction. Partial reconstitution strategy (2.5 mL per cycle) preserves potency of the unreconstituted dry portion within the same vial across multiple reconstitution events.

Sources

  • Source: Heffernan MA et al. — AOD-9604 and adiposity in mice. J Endocrinol. 2001;168(1):1-9
  • Source: Sönksen PH et al. — AOD-9604 Phase 2 visceral fat reduction. J Endocrinol. 2007

Frequently asked questions

Is the 10 mg vial more economical than two 5 mg vials?
The 10 mg vial typically offers better unit pricing and allows the partial reconstitution benefit — the unreconstituted dry powder retains full potency for months, while a reconstituted 5 mg vial begins degrading immediately. For a 12-week protocol at 500 µg/day, three 10 mg vials are needed; you would need six 5 mg vials for the equivalent supply.
Can I use AOD-9604 while on a calorie deficit?
Yes — AOD-9604 is well-suited for use during caloric restriction because it mobilizes stored fat without the muscle-wasting risk associated with severe deficits. The combination of AOD-9604 (fat mobilization) + caloric restriction (energy deficit) + resistance training (muscle preservation) is a frequently used body recomposition stack.
Does AOD-9604 help with cellulite?
AOD-9604 reduces subcutaneous adipocyte size and fat volume, which may improve the surface appearance of cellulite. However, cellulite involves fibrous septae architecture beyond adipocyte volume — AOD-9604 alone will not eliminate structural cellulite. Combination with targeted lipolytic treatments may produce better aesthetic outcomes.
Can AOD-9604 be taken twice daily for faster results?
Some protocols use 250–300 µg twice daily (morning fasted + afternoon before exercise). This maintains lower total dose while extending daily lipolytic signal duration. Once-daily dosing is supported by the strongest clinical data (Phase 2); twice-daily use is off-label.
Does AOD-9604 interact with insulin or thyroid medications?
AOD-9604 does not significantly affect insulin signaling or thyroid axis. Phase 2 data showed no clinically meaningful effect on fasting glucose, insulin, or TSH. No pharmacokinetic interactions with metformin, insulin analogs, or levothyroxine have been identified.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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