MyPeptideMatchHCG Dosing Protocol: 5000 IU Vial — TRT Support & Post-Cycle Therapy Guide
Human chorionic gonadotropin (HCG) 5000 IU vial dosing guide for TRT co-administration, post-cycle therapy, male fertility, and hypogonadism protocols.
Quickstart highlights
HCG (human chorionic gonadotropin) is a 237-amino acid glycoprotein hormone produced by the placental syncytiotrophoblast, structurally homologous to LH.
- Concentration: 5000 IU/mL (5000 IU vial + 1 mL bacteriostatic water).
- At 5000 IU/mL: 250 IU = 0.05 mL, 500 IU = 0.1 mL, 2500 IU = 0.5 mL — use fine-calibration insulin syringe.
- Coviello et al. 2005: 125 IU HCG EOD maintained intratesticular testosterone during testosterone administration.
- Stable 60 days refrigerated after reconstitution — longer than most peptides.
- FDA-approved (Pregnyl, Novarel) for cryptorchidism, male hypogonadism, and female infertility induction.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| TRT support (ongoing) | — | — | Every other day (EOD) | 250 IU EOD — at 5000 IU/mL: draw 0.05 mL; co-administer with TRT to maintain testicular volume and intratesticular testosterone |
| TRT support (alt) | — | — | Twice weekly | 500 IU 2x/week — draw 0.1 mL at 5000 IU/mL; common twice-weekly protocol with TRT |
| PCT (3-week course) | — | — | Twice weekly x 3 weeks | 2500 IU 2x/week — draw 0.5 mL; PCT standard to stimulate testicular recovery post-anabolic cycle |
Reconstitution steps
- Draw 1 mL bacteriostatic water; inject slowly down the vial wall.
- Swirl gently until the lyophilized powder dissolves completely (1–2 minutes); do not shake.
- Final concentration: 5000 IU/mL. At 5000 IU/mL: 250 IU = 0.05 mL, 500 IU = 0.1 mL, 2500 IU = 0.5 mL.
- Label with reconstitution date; refrigerate at 2–8 °C. Use within 60 days. HCG in solution is stable for 60 days refrigerated.
Supplies needed
pct_3wk-week plan
- 2 vials
- 6 syringes
- 2 mL bac water
- 6 alcohol swabs
trt_monthly-week plan
- 1 vial
- 15 syringes
- 1 mL bac water
- 15 alcohol swabs
Protocol overview & cycle notes
Maintain testicular Leydig cell activity, intratesticular testosterone production, and sperm maturation during exogenous testosterone therapy or anabolic steroid post-cycle recovery using regular HCG subcutaneous injections.
Off-cycle: TRT co-administration: continuous (no required off-cycle). PCT: 3-week course then transition to SERM (clomiphene/tamoxifen). Fertility: per physician protocol.
Storage & handling
Injection & tracking tips
- Inject subcutaneously (abdomen or thigh); HCG is not typically injected IM but SC is equally effective and less painful.
- For TRT co-administration: inject HCG on the same days or alternating days as testosterone injections.
- Use an insulin syringe with a 0.5 mL or 1 mL barrel — the small volumes (0.05–0.5 mL) require fine syringe calibration.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Monitor testicular volume (ultrasound or self-assessment) every 8 weeks during TRT co-administration.
- Track LH, FSH, and total testosterone quarterly — HCG suppresses endogenous LH (via negative feedback at pituitary) but maintains testicular Leydig cell function.
- If using for fertility: measure sperm count and motility at baseline and 12 weeks.
How this works & references
Sources
- Low-dose human chorionic gonadotropin maintains intratesticular testosterone in normal men with testosterone-induced gonadotropin suppression. Coviello AD et al. — J Clin Endocrinol Metab — 2005
- Source: Mantovani G et al. — HCG and male hypogonadism: safety and efficacy. J Endocrinol Invest. 2007
- Single and multi-dose pharmacology of recombinant and urinary human chorionic gonadotrophin in men. Handelsman DJ et al. — Clin Endocrinol (Oxf) — 2024
- A randomized three-way cross-over study in healthy pituitary-suppressed women to compare the bioavailability of human chorionic gonadotrophin (Pregnyl) after intramuscular and subcutaneous administration. Mannaerts BM et al. — Hum Reprod — 1998
Frequently asked questions
Why is HCG used during TRT when testosterone already provides androgens?
What is the minimum effective HCG dose for TRT co-administration?
How long should HCG PCT last before starting SERMs?
Does HCG require estrogen management?
Is HCG FDA-approved?
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View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.