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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

Pinealon Dosing Protocol: 20 mg Vial — Neuroprotection & Sleep Regulation Guide

Russian pineal bioregulator Pinealon (Glu-Asp-Arg tripeptide) dosing guide for cognitive protection, circadian rhythm support, and anti-aging neuroprotection.

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Quickstart highlights

Pinealon (Glu-Asp-Arg, EDR tripeptide) is a short peptide bioregulator developed at the St.

  • Concentration: 2 mg/mL (20 mg vial + 10 mL bacteriostatic water).
  • At 2 mg/mL: 200 µg = 10 units per daily injection.
  • One 20 mg vial provides: 100 doses at 200 µg (100 days at once-daily dosing).
  • Russian protocols: 10-day intensive course at 2 mg/day (=10 doses), then maintenance every-other-day.
  • Inject in the evening — supports pineal melatonin synthesis timing.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-220010Once daily (evening)200 µg — 10 units; one 20 mg vial provides 100 doses; standard Russian anti-aging clinic dose for a 10-day intensive course = 2 mg/day
maintenance20010Every other day (long-term)200 µg every other day — 10 units; long-term maintenance for cognitive and circadian support

Reconstitution steps

  1. Draw 10 mL bacteriostatic water in four 2.5 mL draws; inject slowly down the vial wall.
  2. Swirl gently until powder dissolves; do not shake.
  3. Final concentration: 2 mg/mL (20 mg / 10 mL). At 2 mg/mL: 200 µg = 10 units.
  4. Label with date; refrigerate at 2–8 °C. Use within 28 days. Reconstitute in 2.5 mL portions if desired.

Supplies needed

12_wk-week plan

  • 1 vial
  • 42 syringes
  • 5 mL bac water
  • 42 alcohol swabs

10_day-week plan

  • 1 vial
  • 10 syringes
  • 3 mL bac water
  • 10 alcohol swabs
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Protocol overview & cycle notes

Support neuroprotection, melatonin synthesis regulation, and cognitive longevity through Pinealon's pineal gland-targeted tripeptide bioregulator activity, using daily or alternate-day subcutaneous injections.

Off-cycle: Optional off-cycle: 4–8 weeks off after 10-day intensive course; reassess cognitive and sleep quality.

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days. The 20 mg vial provides 100 doses at 200 µg — sufficient for a 10-day intensive (2 mg/day) or 50-day every-other-day protocol.

Injection & tracking tips

  • Inject subcutaneously in the evening — Pinealon's pineal gland affinity supports nighttime melatonin synthesis regulation.
  • Pinealon penetrates the blood-brain barrier based on Russian research; effects on cognition and sleep architecture may take 2–4 weeks to become apparent.
  • May be combined with DSIP (Delta Sleep-Inducing Peptide) for synergistic sleep quality improvement.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Track sleep quality (PSQI score) weekly.
  • Record morning cognitive clarity and memory recall quality (0–10) daily.
  • Monitor melatonin-related parameters: sleep onset time, chronotype stability.
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How this works & references

Pinealon (Glu-Asp-Arg, EDR tripeptide) is a short peptide bioregulator developed at the St. Petersburg Institute of Bioregulation (Khavinson VK). Russian pharmacological research indicates Pinealon penetrates the blood-brain barrier and accumulates in the pineal gland, hypothalamus, and cerebral cortex. Proposed mechanisms: (1) Epigenetic regulation of antioxidant enzyme gene expression in pinealocytes; (2) Restoration of melatonin synthesis capacity in aging pineal tissue; (3) Neuroprotection against oxidative stress via NRF2-like pathways; (4) Modulation of circadian gene expression (BMAL1, Per2). Anisimov et al. and Khavinson's group have published the majority of available data in Russian-language journals.

Sources

  • Source: Khavinson VK et al. — Peptide bioregulation of aging. Ann N Y Acad Sci. 2006;1057:442-60
  • Source: Anisimov VN et al. — Pineal bioregulators and longevity. Aging (Albany NY). 2010

Frequently asked questions

Is Pinealon different from Epithalon (Epitalon)?
Yes — both are Russian bioregulators from Khavinson's group, but from different source tissues: Epithalon (AEDG tetrapeptide) is derived from the epiphysis (pineal gland) cortex; Pinealon (EDR tripeptide) is derived from the internal pineal gland cells. Epithalon has more published research (including telomere-related studies); Pinealon has more circadian and neuroprotective focus.
Can Pinealon replace melatonin supplements?
Pinealon does not directly provide melatonin — it is proposed to restore the pineal gland's capacity to synthesize melatonin in aging tissue. This represents a fundamentally different approach: fixing the gland versus supplementing the hormone. Preliminary Russian data suggests melatonin levels normalize during Pinealon treatment. In practice, combining Pinealon + low-dose melatonin (0.5–1 mg) during the first 4–8 weeks may provide complementary benefits.
Does Pinealon have any side effects?
Russian clinical experience (non-peer-reviewed by Western standards) reports minimal side effects: occasional mild injection site reaction, transient fatigue, and drowsiness (expected from pineal/melatonin-adjacent effects). No serious adverse events have been documented in the available literature.
How is the 10-day intensive course administered?
Russian anti-aging clinic protocols typically use 2 mg/day subcutaneous (or oral/sublingual) for 10 consecutive days, followed by a rest period of 4–8 weeks, then repeating 2–3 times per year. Some practitioners use every-other-day dosing for 20 days to spread the same 10 mg total dose more gradually.
Can Pinealon be taken sublingually instead of injecting?
Russian over-the-counter versions (Cytomaxes) are sold as sublingual tablets. The sublingual route allows some direct absorption without gastrointestinal first-pass degradation. Compounded injectable Pinealon provides higher reliable bioavailability. Both routes are used; the injectable form is preferred for medical protocols.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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