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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

Retatrutide Dosing Protocol: 10 mg Vial — Week-by-Week Titration & Supplies

Titration schedule and supplies for the 10 mg retatrutide compounded vial, covering weeks 1–12 of the standard titration.

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Quickstart highlights

Retatrutide simultaneously activates GLP-1, GIP, and glucagon receptors, producing 24.2% mean body weight reduction at 12 mg weekly in Phase 2 (NCT04881760).

  • Concentration: 5 mg/mL (10 mg vial + 2 mL bacteriostatic water).
  • At 5 mg/mL: 1 mg = 20 units, 4 mg = 80 units, 8 mg = 160 units (requires 3 mL syringe for 1.6 mL volume).
  • Phase 2 trial: 8 mg arm achieved approximately 22% body weight reduction at 48 weeks.
  • One 10 mg vial covers all of weeks 1–4 at 1 mg/dose, with volume remaining for partial week 5–8 dosing.
  • Refrigerate; use within 28 days of reconstitution.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-4100020Once weekly1 mg — 20 units; 10 mg vial provides 10 starting doses
5-8200040Once weekly2 mg — 40 units; 5 doses remaining per vial
9-12400080Once weekly4 mg — 80 units; vial provides ~2.5 doses
13-168000160Once weekly8 mg — 160 units (1.6 mL); use 3 mL syringe; vial provides ~1.25 doses

Reconstitution steps

  1. Draw 2 mL bacteriostatic water; inject slowly down the vial wall.
  2. Swirl gently until fully dissolved; do not shake.
  3. Final concentration: 5 mg/mL (10 mg / 2 mL).
  4. Label with date and concentration; refrigerate at 2–8 °C immediately.

Supplies needed

8-week plan

  • 1 vial
  • 8 syringes
  • 2 mL bac water
  • 8 alcohol swabs

12-week plan

  • 2 vials
  • 12 syringes
  • 4 mL bac water
  • 12 alcohol swabs

16-week plan

  • 3 vials
  • 16 syringes
  • 6 mL bac water
  • 16 alcohol swabs
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Protocol overview & cycle notes

Provide a 12–16 week retatrutide titration cycle using the 10 mg vial as the primary supply, enabling efficient progression from 1 mg to 8 mg maintenance without frequent vial changes.

Cycle length: 16 weeks on.

Off-cycle: 8–12 weeks off between cycles.

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 5 mg/mL: refrigerate 2–8 °C; use within 28 days. Do not freeze.

Injection & tracking tips

  • Use a 3 mL syringe for the 8 mg dose (1.6 mL); a standard 1 mL U-100 syringe is insufficient.
  • Rotate injection sites weekly: abdomen (preferred), outer thigh, upper arm.
  • Drink ≥2.5 L water daily — glucagon agonism increases urinary water loss at higher doses.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Log weekly body weight and fasted waist circumference.
  • Track nausea (0–10 scale) after each injection for the first 12 weeks.
  • Monitor fasting glucose weekly; glucagon effects may transiently elevate fasting blood sugar.
Log your cycle in the calculator →

How this works & references

Retatrutide simultaneously activates GLP-1, GIP, and glucagon receptors, producing 24.2% mean body weight reduction at 12 mg weekly in Phase 2 (NCT04881760). The 8 mg dose arm achieved ~22% weight loss at 48 weeks. MASH improvement and cardiometabolic risk reduction are being evaluated in Phase 3 TRIUMPH trials.

Sources

Frequently asked questions

How do I draw 1.6 mL from the vial for the 8 mg dose?
Use a 3 mL syringe instead of a 1 mL insulin syringe. Draw to the 1.6 mL line. At 5 mg/mL concentration, 1.6 mL = 8 mg. Never use an insulin syringe for volumes exceeding 1 mL.
When will retatrutide be FDA-approved?
FDA approval is anticipated no earlier than 2027, pending completion of Phase 3 TRIUMPH trials. As of February 2026, it remains in Phase 3 testing only.
Is the 10 mg vial the best size for the full titration?
The 10 mg vial is ideal for weeks 1–12 of the standard titration. For maintenance doses of 8–12 mg, the 20 mg or 30 mg vials are more efficient, requiring fewer reconstitutions.
What distinguishes retatrutide from semaglutide and tirzepatide?
Retatrutide adds glucagon receptor agonism to GLP-1 and GIP receptor activity. The glucagon component drives hepatic fat oxidation and thermogenesis, explaining retatrutide's superior weight loss compared to GLP-1-only and dual GLP-1/GIP agents in comparable trial populations.
Does retatrutide cause thyroid cancer risk like GLP-1 agonists?
The same black box warning applies: avoid in personal or family history of medullary thyroid carcinoma (MTC) or multiple endocrine neoplasia type 2 (MEN2). GLP-1 receptor activation has been associated with thyroid C-cell hyperplasia in rodents; human clinical relevance is under study.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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