MyPeptideMatchSermorelin Dosing Protocol: 5 mg Vial — Daily Dosing Schedule & Supplies
Week-by-week subcutaneous dosing schedule for sermorelin GHRH analogue using the 5 mg vial, with reconstitution steps and supplies.
Quickstart highlights
Sermorelin acetate (GHRH 1-29 NH2) is a 29-amino acid analogue of endogenous growth hormone-releasing hormone (GHRH) that binds pituitary GHRH receptors with high affinity, stimulating pulsatile GH release through a physiological mechanism rather than direct GH administration.
- Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
- At 2 mg/mL: 100 µg = 5 units, 200 µg = 10 units, 300 µg = 15 units, 500 µg = 25 units.
- Inject nightly 30–60 minutes before sleep; avoid high-glycemic food 2 hours before.
- One 5 mg vial provides 25 nightly doses at 200 µg or ~17 doses at 300 µg.
- Combine with ipamorelin or GHRP-2 for synergistic GH secretion via complementary receptors.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| 1-4 | 200 | 10 | Once daily (before bed) | 200 µg nightly — 10 units; one 5 mg vial provides 25 nightly doses |
| 5-12 | 300 | 15 | Once daily (before bed) | 300 µg — 15 units; often combined with GHRP (ipamorelin/GHRP-2) for synergy |
| 13+ | 500 | 25 | Once daily (before bed) | 500 µg maintenance — 25 units; maximum clinical dose in most protocols |
Gradual approach
| Week | Dose (µg) | Units | Notes |
|---|---|---|---|
| 1-8 | 100 | 5 | 100 µg conservative start — ideal for sensitive individuals or first GH peptide cycle |
| 9-16 | 200 | 10 | Increase to 200 µg if 100 µg well-tolerated |
Reconstitution steps
- Draw 2.5 mL bacteriostatic water into a 3 mL syringe; inject slowly down the vial wall.
- Swirl gently until the lyophilized powder fully dissolves (1–2 minutes); do not shake.
- Final concentration: 2 mg/mL (2,000 µg/mL). At this concentration, 1 unit on a U-100 syringe = 20 µg.
- Label with date and concentration; refrigerate at 2–8 °C. Use within 20 days (based on 25 doses × 200 µg from 5 mg vial).
Supplies needed
8-week plan
- 1 vial
- 56 syringes
- 3 mL bac water
- 56 alcohol swabs
12-week plan
- 2 vials
- 84 syringes
- 5 mL bac water
- 84 alcohol swabs
Protocol overview & cycle notes
Restore physiological GH pulsatility through GHRH receptor stimulation with nightly sermorelin injections, targeting improved body composition, recovery, and sleep quality over a 12-week cycle.
Cycle length: 12 weeks on.
Off-cycle: 4–6 weeks off after every 12-week cycle; reassess IGF-1 levels before restarting.
Storage & handling
Injection & tracking tips
- Inject subcutaneously 30–60 minutes before sleep — sermorelin acts on the hypothalamic-pituitary axis; endogenous GH pulsatility peaks during early sleep stages (NREM Stage 3).
- Avoid food or high blood glucose for 2 hours before injecting; elevated insulin blunts GH secretion.
- If combining with a GHRP (ipamorelin, GHRP-2), inject both at the same time from separate syringes at adjacent sites.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Measure IGF-1 serum level at baseline, 6 weeks, and 12 weeks to quantify GH axis response.
- Track sleep quality (subjective 0–10 and wearable deep sleep duration) — sermorelin typically improves delta-wave sleep within 4–6 weeks.
- Record body composition changes (waist circumference, lean mass via InBody) every 8 weeks.
How this works & references
Sources
- Source: Vittone J et al. — Sermorelin 500 µg nightly increases IGF-1 in GH-deficient adults. J Clin Endocrinol Metab. 1997
- FDA — Geref (sermorelin acetate for injection) prescribing information
- Polyethylene glycol-conjugated growth hormone-releasing hormone is long acting and stimulates GH in healthy young and elderly subjects. Munafo A et al. — Eur J Endocrinol — 2005
Frequently asked questions
Why inject sermorelin before bed?
Should I combine sermorelin with a GHRP?
How long before I see results from sermorelin?
Is sermorelin FDA-approved?
Can I use sermorelin long-term without a break?
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View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.