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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

Epithalon (Epitalon) Dosing Protocol: 10 mg Vial — Telomere & Anti-Aging Guide

Epithalon AEDG tetrapeptide anti-aging protocol — 10-day intensive course for telomerase activation, melatonin synthesis restoration, and longevity optimization.

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Quickstart highlights

Epithalon (Ala-Glu-Asp-Gly, AEDG) is a synthetic tetrapeptide developed by Vladimir Khavinson at the St.

  • Concentration: 2 mg/mL (10 mg vial + 5 mL bacteriostatic water).
  • At 2 mg/mL: 10 mg/day = 500 units — use a 3 mL syringe; divide into five 100-unit (1 mL) insulin syringe draws.
  • Standard protocol: 10 mg once daily x 10 consecutive days (1 vial = 1 complete course).
  • Khavinson et al. 2003: AEDG telomerase induction in human fetal fibroblasts at nanomolar concentrations.
  • Repeat course 2–3x per year (every 4–6 months); inject in the evening for circadian support.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-2 (10 days)10000500Once daily (evening)10 mg — 500 units on a U-100 syringe; one 10 mg vial provides exactly 1 complete 10-day intensive course

Reconstitution steps

  1. Draw 5 mL bacteriostatic water (two 2.5 mL draws); inject slowly down the vial wall.
  2. Swirl gently until dissolved; do not shake.
  3. Final concentration: 2 mg/mL. At 2 mg/mL: 10 mg = 500 units — use a 1 mL (100-unit) insulin syringe filled to 100 units, withdrawing 5 separate 100-unit draws, OR use a 3 mL syringe.
  4. Label with date; refrigerate at 2–8 °C. Use within 28 days.

Supplies needed

10_day_course-week plan

  • 1 vial
  • 10 syringes
  • 5 mL bac water
  • 10 alcohol swabs
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Protocol overview & cycle notes

Activate telomerase, restore pineal melatonin synthesis, reduce oxidative stress, and extend cellular lifespan through a 10-day intensive Epithalon subcutaneous course, repeated 2–3 times annually.

Off-cycle: 4–6 months between 10-day intensive courses (2–3 courses per year is standard).

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days. The full 10 mg vial is used in a single 10-day course at 10 mg/day.

Injection & tracking tips

  • Inject subcutaneously in the evening — Epithalon's pineal gland affinity supports melatonin synthesis restoration during nighttime.
  • Use a 3 mL syringe for the 5 mL volume, or divide into five 1 mL insulin syringe draws at 100 units per draw for the daily 10 mg dose.
  • The 10-day consecutive daily course is the standard protocol; do not extend beyond 10 consecutive days without medical guidance.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Obtain telomere length testing (e.g., Life Length, Telomere Diagnostics) before and 3 months after each course to assess response.
  • Track melatonin-related markers: sleep quality (PSQI), sleep onset time, and morning energy.
  • Log inflammatory biomarkers (hsCRP, IL-6) at baseline and 3 months post-course.
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How this works & references

Epithalon (Ala-Glu-Asp-Gly, AEDG) is a synthetic tetrapeptide developed by Vladimir Khavinson at the St. Petersburg Institute of Bioregulation. It induces telomerase (hTERT) expression in human fetal fibroblasts, extending replicative lifespan in culture (Khavinson et al., 2003). Additional mechanisms: (1) Restoration of melatonin synthesis in aging pinealocytes; (2) Normalization of cortisol and gonadotropin rhythms; (3) Upregulation of antioxidant enzymes (SOD, catalase, glutathione peroxidase); (4) DNA repair enzyme activation. In a 25-year longitudinal cohort study in Russian clinical settings, Epithalon treatment was associated with 1.6-fold lower mortality versus untreated controls (Anisimov et al., 2011).

Sources

Frequently asked questions

Does Epithalon really activate telomerase in humans?
Khavinson et al. (2003) demonstrated telomerase activity induction in human fetal fibroblast cell cultures at nanomolar AEDG concentrations. Human in vivo telomerase activation data is indirect — from observational studies showing reduced mortality and biomarker improvements in treated cohorts. No RCT with telomere length as primary outcome has been published as of 2026.
How often should I repeat the 10-day Epithalon course?
Standard Russian bioregulator protocols repeat the 10-day intensive course 2–3 times per year (every 4–6 months). Some longevity-focused practitioners use shorter courses (5 days at 10 mg/day) every 3 months for more continuous support. The total annual dose in these protocols ranges from 100–200 mg.
Is Epithalon the same as Epitalon?
Yes — Epithalon and Epitalon are identical compounds (Ala-Glu-Asp-Gly, AEDG tetrapeptide). The naming difference reflects transliteration conventions: Epithalon (Russian/formal) vs. Epitalon (Western anglicization). Chemical structure and biological activity are identical.
Can Epithalon be combined with Pinealon?
Yes — Epithalon and Pinealon are complementary Russian bioregulators with overlapping pineal gland affinity. Some longevity protocols combine them: Epithalon (AEDG) for telomerase activation and melatonin synthesis restoration, Pinealon (EDR) for neuroprotection and circadian rhythm support. Sequential or concurrent daily injections during the 10-day course are used.
What blood tests should I track during Epithalon protocols?
Key biomarkers: (1) Telomere length (commercial testing, before and 3 months post-course); (2) IGF-1 (may increase modestly); (3) Melatonin DLMO (dim-light melatonin onset) for circadian normalization; (4) hsCRP and IL-6 for inflammatory burden; (5) CBC and comprehensive metabolic panel for safety monitoring.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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