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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

Epithalon (Epitalon) Dosing Protocol: 20 mg Vial — Two-Course Annual Supply Guide

Epithalon 20 mg vial guide covering two complete 10-day intensive courses (10 mg/day each), ideal for semi-annual anti-aging protocols.

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Quickstart highlights

The 20 mg vial allows two complete Epithalon 10-day intensive courses using partial reconstitution.

  • Concentration: 2 mg/mL per reconstitution (reconstitute 5 mL at a time, = 10 mg per reconstitution).
  • One 20 mg vial = 2 complete 10-day courses at 10 mg/day.
  • Partial reconstitution: reconstitute 5 mL for Course 1; remaining 10 mg dry powder stores safely for months.
  • Standard protocol: twice-yearly courses (spring + autumn) at 10 mg/day x 10 days.
  • Inject in the evening; use 3 mL syringe for 5 mL injection volume.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
—10000500Once daily x 10 days (evening)10 mg — 500 units; reconstitute first 5 mL (half the vial) for course 1
—10000500Once daily x 10 days (evening)Reconstitute remaining 5 mL for course 2 — the dry peptide retains full potency until second reconstitution

Reconstitution steps

  1. For Course 1: Draw 5 mL bacteriostatic water; inject into the 20 mg vial slowly down the wall.
  2. This reconstitutes 10 mg at 2 mg/mL — sufficient for a 10-day course at 10 mg/day.
  3. Store reconstituted vial at 2–8 °C; use within 28 days.
  4. For Course 2 (months later): The remaining dry peptide powder remains in the vial — draw another 5 mL bac water and reconstitute the second 10 mg portion.

Supplies needed

per_course-week plan

  • 0.5 vials
  • 10 syringes
  • 5 mL bac water
  • 10 alcohol swabs

annual_two_courses-week plan

  • 1 vial
  • 20 syringes
  • 10 mL bac water
  • 20 alcohol swabs
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Protocol overview & cycle notes

Execute two semi-annual 10-day Epithalon intensive courses (spring and autumn) from a single 20 mg vial using partial reconstitution, for cost-efficient long-term telomere maintenance and anti-aging support.

Off-cycle: 4–6 months between each 10-day intensive course; 2 courses per year from one 20 mg vial.

Storage & handling

Lyophilized: store below 25 °C. The 20 mg vial is designed for partial reconstitution: reconstitute 5 mL (10 mg) for each course. The unreconstituted dry portion in the vial remains stable at room temperature until needed. Reconstituted solution: refrigerate 2–8 °C, use within 28 days.

Injection & tracking tips

  • Perform the second reconstitution of the remaining dry powder no earlier than 4 months after the first course, to respect the 28-day use window of the second reconstitution.
  • Label the vial with Course 1 reconstitution date; mark when Course 2 is planned.
  • The two-course-per-year schedule (spring + autumn) aligns with traditional Russian bioregulator protocols.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Test telomere length before the first course and 6 months post-second course for long-term assessment.
  • Track melatonin quality indicators (sleep latency, depth) around each course.
  • Monitor IGF-1 and DHEA-S annually as longevity biomarkers.
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How this works & references

The 20 mg vial allows two complete Epithalon 10-day intensive courses using partial reconstitution. Lyophilized peptide stability is substantially greater than solution stability — the unreconstituted portion of the vial retains potency for months to years when stored dry below 25 °C. This architecture reduces cost and number of vials needed for annual protocols. The spring-autumn 6-month cycle mirrors seasonal longevity protocols used in Russian clinical practice.

Sources

Frequently asked questions

Is it safe to partially reconstitute a lyophilized peptide vial?
Yes — lyophilized (freeze-dried) peptides are stable as a dry powder for months to years below 25 °C. Partial reconstitution is well-established practice for this reason. Draw only the volume of bacteriostatic water needed for the first course (5 mL for 10 mg at 2 mg/mL), use within 28 days, then reconstitute the remaining dry powder months later.
Should the 20 mg vial be stored differently before the second reconstitution?
After the first reconstitution of 5 mL, the remaining dry powder in the vial is exposed to the refrigerator environment. For best preservation: after the first course, seal the vial with a new stopper crimp if possible, or store in a sealed zip-lock bag with a desiccant packet at room temperature (not refrigerator — condensation can degrade residual dry powder). Freeze at -20 °C for maximum preservation between courses.
Do I need two separate vials for a twice-yearly protocol?
No — a single 20 mg vial covers two complete 10-day courses at 10 mg/day. The partial reconstitution strategy makes the 20 mg vial the most cost-efficient choice for semi-annual protocols. For quarterly protocols (4 courses per year), you would need two 20 mg vials annually.
Is there a lower-dose option for Epithalon?
Yes — some practitioners use 5 mg/day for 20 days (instead of 10 mg/day for 10 days), giving the same 100 mg total course dose with a gentler daily administration. At 5 mg/day from a 2 mg/mL solution, the injection volume is 2.5 mL — more manageable with a 3 mL syringe.
What evidence supports twice-yearly versus once-yearly Epithalon courses?
No head-to-head RCT comparing annual frequencies exists. Russian clinical protocols empirically developed the 2–3 courses/year schedule based on observed biomarker improvement patterns. Twice-yearly is considered a reasonable balance between continuous telomerase support and cost/convenience.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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