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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

TB-500 Dosing Protocol: 10 mg Vial — Extended Loading & Maintenance Guide

TB-500 10 mg vial guide providing a full 2.5-week loading supply at 2 mg 2x weekly from a single reconstitution.

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Quickstart highlights

TB-500 Thymosin Beta-4 fragment promotes angiogenesis, stem cell migration, and inflammation resolution through ILK (Integrin-Linked Kinase) and BMP-4 pathways (Bock-Marquette et al., 2004).

  • Concentration: 2 mg/mL (10 mg vial + 5 mL bacteriostatic water).
  • At 2 mg/mL: 2 mg = 100 units per loading injection.
  • One 10 mg vial provides 5 loading doses (2.5 weeks at 2x weekly) or 5 maintenance doses (10 weeks at biweekly).
  • Never shake TB-500 — mechanical agitation degrades the peptide structure.
  • Refrigerate; use within 28 days.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-62000100Twice weekly2 mg 2x weekly — 100 units each; 10 mg vial provides 5 loading injections (~2.5 weeks)
7-122000100Once every 2 weeks2 mg maintenance — one 10 mg vial provides 5 maintenance doses (10 weeks)

Reconstitution steps

  1. Draw 5 mL bacteriostatic water (two 2.5 mL draws); inject slowly and gently down the vial wall.
  2. Swirl gently — never shake TB-500; mechanical agitation degrades the peptide.
  3. Final concentration: 2 mg/mL. Label with date; refrigerate at 2–8 °C. Use within 28 days.

Supplies needed

12-week plan

  • 3 vials
  • 18 syringes
  • 15 mL bac water
  • 18 alcohol swabs
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Protocol overview & cycle notes

Provide an efficient 2.5-week loading supply or 10-week maintenance supply from the 10 mg vial for the TB-500 tissue repair protocol, with fewer reconstitution events than the 5 mg vial.

Cycle length: 12 weeks on.

Off-cycle: 4–8 weeks off after 12-week cycle.

Storage & handling

Lyophilized: store below 25 °C. Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days. Handle gently — never shake.

Injection & tracking tips

  • The 10 mg vial at 2 mg/mL provides 5 doses of 2 mg each — covering 2.5 weeks of 2x-weekly loading.
  • For the full 6-week loading phase (12 injections at 2 mg): plan 2.4 vials (3 vials with some remainder).
  • The 10 mg vial is ideal for maintenance phase — one vial provides 5 maintenance doses (2 mg every 2 weeks = 10 weeks).

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Rate injured tissue pain and mobility weekly during loading phase.
  • Track systemic inflammation markers (if available: hsCRP, fibrinogen) at baseline and 6 weeks.
  • Log exercise capacity, training performance, and injury impact on daily activities weekly.
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How this works & references

TB-500 Thymosin Beta-4 fragment promotes angiogenesis, stem cell migration, and inflammation resolution through ILK (Integrin-Linked Kinase) and BMP-4 pathways (Bock-Marquette et al., 2004). Smart et al. (2007) demonstrated TB-4 mobilizes epicardial progenitor cells for cardiac vascular repair in mouse models. The 10 mg vial offers 5 × 2 mg doses from a single reconstitution, reducing sterility risks over the 28-day use window.

Frequently asked questions

How many 10 mg vials do I need for a full 6-week loading + 6-week maintenance cycle?
Loading (6 weeks x 2 doses/week x 2 mg = 24 mg): 2.4 vials (3 vials). Maintenance (6 weeks x 1 dose/2 weeks x 2 mg = 6 mg): 0.6 vials (1 vial). Total: approximately 4 vials for a full 12-week protocol.
Can I use a 5 mg dose of TB-500 instead of 2 mg?
Some advanced protocols use 5 mg 2x weekly (veterinary TB-500 literature uses this dose range in horses). Human protocols typically stay at 2–4 mg 2x weekly, as higher doses have not demonstrated proportionally greater benefit in available data and cost increases significantly.
Is TB-500 effective for hair loss?
Animal and in vitro data suggest Thymosin Beta-4 promotes hair follicle stem cell activation and hair growth. Some anecdotal reports exist for TB-500 improving hair density in humans. No controlled human trials are published for this indication.
What injection needle gauge is recommended for TB-500?
A 29G–31G, 4–6 mm subcutaneous needle is standard. TB-500 is administered SC (not IM), and the fine gauge minimizes discomfort. The 2 mg dose (100 units = 1 mL) may be slightly uncomfortable for some patients with 31G needles due to higher pressure needed for 1 mL volume — a 29G may be preferable for the full 1 mL dose.
How is TB-500 detected in anti-doping testing?
TB-500 / Thymosin Beta-4 is detectable via LC-MS/MS peptide profiling in plasma or urine samples. The detection window is estimated at 24–72 hours post-subcutaneous injection, though this may vary by dose and laboratory method.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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