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Reviewed by MyPeptideMatch Editorial TeamLast reviewed February 2026Updated February 2026

TB-500 Dosing Protocol: 5 mg Vial — Loading & Maintenance Schedule

Thymosin Beta-4 (TB-500) dosing guide for the 5 mg vial — loading and maintenance phases for systemic tissue repair and anti-inflammatory effects.

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Quickstart highlights

TB-500 is a synthetic peptide corresponding to the active fragment (Ac-SDKP, 17 amino acids from the C-terminus) of Thymosin Beta-4 — a 43-amino acid actin-sequestering protein ubiquitous in human tissue.

  • Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
  • At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
  • Loading phase: 2 mg 2x weekly for 4–6 weeks; maintenance: 2 mg every 2 weeks.
  • TB-500 acts systemically — inject in abdomen, not at injury site.
  • One 5 mg vial provides ~2.5 loading doses (2 mg each); plan 4 vials for a 6-week loading phase.

Dosing table

For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.

WeekDose (µg)UnitsFrequencyNotes
1-62000100Twice weekly2 mg 2x weekly — 100 units each; loading phase; one 5 mg vial provides ~2.5 injections
7+2000100Every 2 weeks2 mg every 2 weeks — maintenance phase; one 5 mg vial provides 2 maintenance doses

Gradual approach

WeekDose (µg)UnitsNotes
1-81000501 mg 2x weekly — conservative loading; suitable for sensitive patients or first TB-500 cycle
9-122000100Advance to 2 mg 2x weekly if 1 mg well-tolerated

Reconstitution steps

  1. Draw 2.5 mL bacteriostatic water; inject slowly down the vial wall.
  2. Swirl gently until lyophilized powder dissolves; do not shake — TB-500 is susceptible to degradation from mechanical agitation.
  3. Final concentration: 2 mg/mL. At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
  4. Label with date; refrigerate at 2–8 °C. Use within 28 days.

Supplies needed

8-week plan

  • 4 vials
  • 16 syringes
  • 10 mL bac water
  • 16 alcohol swabs

12-week plan

  • 7 vials
  • 24 syringes
  • 18 mL bac water
  • 24 alcohol swabs
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Protocol overview & cycle notes

Promote systemic tissue repair, reduce inflammation, and accelerate injury recovery through the TB-500 loading-then-maintenance protocol, leveraging Thymosin Beta-4's unique actin-sequestering and stem cell-mobilizing properties.

Cycle length: 12 weeks on.

Off-cycle: 4–8 weeks off after 12-week cycle.

Storage & handling

Lyophilized: store below 25 °C (or refrigerate). Reconstituted at 2 mg/mL: refrigerate 2–8 °C; use within 28 days. TB-500 is a larger peptide than BPC-157 — handle gently; avoid shaking or vortexing.

Injection & tracking tips

  • TB-500 has systemic effects unlike BPC-157 — injection site does not need to be near the injury; abdomen is preferred for reliable subcutaneous absorption.
  • Do not shake the reconstituted vial; TB-500 (as a fragment of the 43-amino acid Thymosin Beta-4) is more prone to mechanical degradation than smaller peptides.
  • For combination with BPC-157: use separate syringes and separate injection sites; TB-500 and BPC-157 can be used on the same days.

Tracking

Logging helps you and your provider spot patterns and adjust dose or timing.

  • Track injury pain (0–10 VAS) and range of motion weekly during loading phase.
  • Log systemic effects: fatigue levels, exercise recovery, and any soft tissue inflammation indicators.
  • Measure inflammatory markers (CRP, ESR) at baseline and after 6 weeks if clinically indicated.
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How this works & references

TB-500 is a synthetic peptide corresponding to the active fragment (Ac-SDKP, 17 amino acids from the C-terminus) of Thymosin Beta-4 — a 43-amino acid actin-sequestering protein ubiquitous in human tissue. TB-500's tissue repair mechanisms include: (1) G-actin sequestration promoting cell migration and cytoskeletal organization; (2) upregulation of cell surface receptor LIMS1 (LIM domain) for stem cell mobilization; (3) BMP-4 and Wnt pathway activation for cardiac and blood vessel repair (Bock-Marquette et al., 2004). Unlike BPC-157, TB-500 acts systemically rather than locally and does not require injection at the injury site.

Frequently asked questions

What is the difference between TB-500 and Thymosin Beta-4?
Thymosin Beta-4 (Tβ4) is the full 43-amino acid protein; TB-500 is the active fragment corresponding to amino acids 17–23 of Tβ4 (Ac-LKKTETQ in some definitions, though commercial 'TB-500' typically refers to the entire peptide sequence used in veterinary and research studies). The therapeutic properties of the commercially available TB-500 mimic those of full Tβ4 in animal models.
Should I inject TB-500 near the injury, like BPC-157?
No — this is a key distinction. TB-500 works systemically via bloodstream-mediated mechanisms (stem cell mobilization, systemic actin regulation). Any subcutaneous site (abdomen preferred) provides equivalent absorption and distribution. BPC-157 benefits from local injection near the injury; TB-500 does not require this.
How long does the loading phase need to be?
Most protocols use a 4–6 week loading phase at 2 mg 2x weekly (total 16–24 mg). Shorter loading (4 weeks) is appropriate for mild injuries; 6 weeks for chronic or severe injuries. After loading, the maintenance dose of 2 mg every 2 weeks sustains therapeutic tissue levels.
Can TB-500 and BPC-157 be used simultaneously?
Yes — this is one of the most common peptide combinations in injury recovery. BPC-157 provides local angiogenic and FAK-mediated repair, while TB-500 provides systemic stem cell mobilization and actin-cytoskeletal support. Use separate syringes; inject at separate abdominal sites or on the same or alternating days.
Is TB-500 WADA-prohibited?
Yes — Thymosin Beta-4 and its fragments are listed under WADA S2 (Peptide Hormones, Growth Factors, Related Substances). The peptide is detectable in plasma for approximately 24–48 hours after subcutaneous injection using LC-MS/MS methods.

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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.

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