TB-500 Dosage Guide: How Much Should You Take? (2026)

Key Takeaways

• Standard Dosage Range: TB-500 is typically administered at 2-5 mg per week via subcutaneous injection.
• Administration Method: Most commonly delivered through subcutaneous injection, though other routes are under research.
• Typical Cycle Length: Initial cycles often last 4-6 weeks, followed by maintenance dosing.
• Regulatory Status: TB-500 is not FDA-approved for human use and is primarily used in research settings.
• Medical Supervision: Always consult with a healthcare provider experienced in peptide therapy before starting TB-500.

How TB-500 Works in the Body

TB-500, a synthetic derivative of thymosin beta-4, is known for its tissue repair and anti-inflammatory properties. Its mechanism of action involves binding to actin, a cellular protein crucial for cell movement and structure. By promoting actin polymerization, TB-500 enhances cell migration and wound healing processes.

The peptide’s half-life and bioavailability significantly influence dosing schedules. TB-500 has a relatively short half-life, necessitating frequent dosing to maintain therapeutic levels. Its bioavailability varies depending on the administration route, with subcutaneous injections being the most effective.

"TB-500's efficacy in promoting angiogenesis and reducing inflammation has been demonstrated in several preclinical models, highlighting its potential in tissue regeneration applications." (PMID 41490200)

Standard TB-500 Dosage Ranges (By Form)

Subcutaneous Injection

• Dosage Range: 2-5 mg per week, divided into 2-3 injections.
• Frequency: Administered every 3-4 days.
• Bioavailability: High, making it the preferred method for therapeutic use.
• Common Usage: Most clinics and studies use this form due to its effectiveness and ease of administration.

Intramuscular Injection

• Dosage Range: Similar to subcutaneous, but less common due to potential discomfort.
• Frequency: Weekly or bi-weekly.
• Bioavailability: Comparable to subcutaneous but less frequently used in practice.

TB-500 Dosage by Use Case

Tissue Repair and Recovery

• Dosage: 2-5 mg per week.
• Administration: Subcutaneous injection.
• Cycle Length: 4-6 weeks, followed by maintenance dosing.
• Study Reference: "TB-500 has shown promise in enhancing recovery in musculoskeletal injuries." (PMID 41476424)

Anti-Inflammation

• Dosage: 2-4 mg per week.
• Administration: Subcutaneous injection.
• Cycle Length: 4 weeks, with possible extension based on clinical response.
• Clinical Protocols: Many clinics prescribe TB-500 for its anti-inflammatory effects, especially in conditions like tendinitis.

Wound Healing

• Dosage: 3-5 mg per week.
• Administration: Subcutaneous injection.
• Cycle Length: 6 weeks or until wound closure.
• Clinical Reference: "TB-500 accelerates wound healing by promoting cellular migration and angiogenesis." (PMID 23084823)

How Long Should You Take TB-500?

Typical TB-500 cycles last between 4 to 6 weeks, depending on the therapeutic goal. Initial loading phases may involve higher doses, transitioning to a maintenance phase with reduced frequency. Tapering is generally not required, but stopping TB-500 may lead to a gradual return to baseline inflammation or healing rates. Data on long-term use is limited and primarily derived from animal studies.

Administration Protocol: How to Take TB-500

Injection Technique

• Needle Gauge: Use a 29-31 gauge insulin syringe.
• Injection Sites: Rotate between abdomen, thigh, and upper arm to minimize tissue irritation.
• Rotation Schedule: Change injection site with each administration.

Storage Requirements

• Temperature: Store lyophilized powder in a cool, dry place; reconstituted solution should be refrigerated.
• Light Sensitivity: Protect from light exposure to preserve peptide integrity.
• Reconstitution: Follow reconstitution guide for detailed instructions.

Step-by-Step Process

1. Reconstitute the peptide with bacteriostatic water.
2. Draw the required dose into the syringe.
3. Clean the injection site with an alcohol swab.
4. Inject subcutaneously at a 45-degree angle.
5. Dispose of the syringe safely.

Stacking TB-500 for Better Results

TB-500 and BPC-157

• Combination: Enhances tissue repair and reduces recovery time.
• Dosing Synergy: TB-500 at 2-5 mg/week, BPC-157 at 200-500 mcg/day.
• Common Protocols: Often used in musculoskeletal injury recovery.

TB-500 and Growth Hormone Secretagogues

• Combination: Promotes overall recovery and tissue regeneration.
• Dosing Synergy: TB-500 at 2-5 mg/week, combined with peptides like ipamorelin.
• Protocol Names: Sometimes referred to as part of the "Wolverine protocol."

Factors That Affect Your TB-500 Dosage

Dosage adjustments may be necessary based on body weight, age, treatment goals, and individual response. Kidney or liver function can also influence metabolism and clearance of the peptide. Healthcare providers tailor dosing to each patient’s unique physiology and therapeutic needs.

Common Dosing Mistakes to Avoid

• Dosing Too High/Too Low: Follow established protocols to avoid adverse effects or suboptimal outcomes.
• Inconsistent Timing: Maintain regular dosing schedules to ensure consistent therapeutic levels.
• Wrong Injection Site: Use recommended sites for effective absorption.
• Regulatory Status: Be aware of TB-500's research status and legal implications.
• Poor Storage/Handling: Proper storage is crucial to maintain peptide stability and efficacy.

What the Evidence Does Not Show

While preclinical studies suggest promising benefits, long-term safety data in humans is lacking. Most evidence derives from animal models, and human trials are limited. There is also a need for more dose-response studies to refine optimal dosing strategies.

FAQ — Your Top TB-500 Dosage Questions Answered

Best time of day to take it?
TB-500 can be administered at any time, though consistency is key. Many prefer morning injections.

What if you miss a dose?
Administer as soon as you remember, but do not double up doses.

Can men and women use the same dose?
Yes, dosing is generally based on therapeutic goals rather than gender.

Can you stack it with other peptides?
Yes, TB-500 is often stacked with peptides like BPC-157 for enhanced effects.

How long until you see results?
Patients may notice improvements within 2-4 weeks, depending on the condition treated.

Do you need to cycle off?
Cycling off is not mandatory but may be considered to assess baseline status.

Is a loading dose needed?
Some protocols start with a higher loading dose to rapidly achieve therapeutic levels.

How to adjust dose if side effects occur?
Consult with a healthcare provider to potentially reduce the dose or adjust the administration schedule.

Conclusion — Finding the Right Dosage for You

Determining the appropriate TB-500 dosage involves careful consideration of individual factors and therapeutic goals. Always consult a healthcare provider experienced in peptide therapy before beginning treatment. For more information, explore our clinic finder and related peptide guides on BPC-157 and ipamorelin.

Medical Disclaimer: This content is for informational purposes only and does not constitute medical advice. Dosage information is compiled from published research and clinical protocols. Consult a licensed healthcare provider before starting any peptide therapy. Use our clinic finder to locate a qualified provider near you.